Cisplatin a549 ic50

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August undefined, 2024

WebNov 29, 2024 · This study revealed that cisplatin resistant A549 lung cancer cells can be identified by their increased levels of mitochondrial mass. However, Mito-High cells … WebCisplatin (DDP) has been a significant tumor chemotherapy against various solid malignant tumors, including lung adenocarcinoma, since it was approved by the Food and Drug …

Inhibitory concentrations 50 (IC50) and CI95 for cisplatin and …

WebThe 50 % inhibitory concentration (IC50) of cisplatin in A549 and A549/DDP cells 48 h after drug treatment Source publication +4 RNA interferences targeting the Fanconi … WebIn A549 cells, the IC50 concentration of cisplatin resistant cells was determined as 23.60 µM compared to 1.58 µM in the original parent cell line, a 15-fold increase in the … simple ground turkey recipes

Determination of IC50 values of anticancer drugs on cells by D2O ...

WebApr 13, 2024 · Cisplatin‑resistant A549 (A549/CDDP) and H460 (H460/CDDP) cells were first established and were then transfected with negative control small interfering (si)RNA (si‑NC) or TRPC1 siRNA (si‑TRPC1). ... TRPC1 silencing decreased the IC50 value of CDDP compared with the si‑NC group in A549/CDDP (11.78 vs. 21.58 µM; P<0.01) and … WebThe cisplatin sensitivity of A549/DDP by the MTT assay, IC50 = 2.2μg/mL. The cisplatin sensitivity of A549 by the MTT assay, IC50 = 0.8μg/mL (B) Wound healing assays were … WebFeb 3, 2024 · A549 and A549/CDDP cells were treated with 1/2 IC50 of cisplatin and/or PD‐0332991 for 24 h. Then cells were homogenized in RIPA buffer (50 mM Tris–HCl, pH … rawlings university florida

Cisplatin resistance in non-small cell lung cancer cells is …

Cisplatin Induces a Mitochondrial-ROS Response That Contributes …

WebApr 11, 2024 · Cisplatin-resistant mRNA and miRNA models were constructed by Logistic regression, classification and regression tree and C4.5 decision tree classification algorithm with previous feature selection performed via least absolute shrinkage and selection operator (LASSO). qRT-PCR and western-blotting of A549 and H358 cells, as well as … WebOct 1, 2024 · Cisplatin-resistant A549 cells (A549/CisR) were derived from A549 cells by continuous cisplatin treatment. The original parental A549 cells were initially treated … rawlings velo 2.0 catcher\u0027s gearWebImmunofluorescence results elucidated that cellular ROS level in Cisplatin-induced A549 cells increases in a dose-dependent manner. Both immunofluorescence and flow cytometry results revealed that the apoptotic rate of A549 cells increases with the elevation of Cisplatin dose. rawlings velo 2.0 catcher\\u0027s gear

"WebApr 11, 2024 · Obviously, the resistance to cisplatin varies among different cancers. In the Depmap database, we successfully classify different tumor cell lines according to their source of cancer, so as to obtain the average IC50 value of cisplatin of related cancers. " - Cisplatin a549 ic50

Cisplatin a549 ic50

Cisplatin-resistant HeLa cells are resistant to apoptosis via p53 ...

WebJul 23, 2015 · The IC50 of cisplatin for A549 and SPC-A1 cells under hypoxia was 3.38-fold and 1.57-fold higher as compared to that under normoxia respectively as showing by the … WebJan 17, 2024 · A simple, sensitive and repeatable D2O-single cell Raman spectroscopy method is developed to quantify the inhibitory activity of anticancer drugs on cancer cell …

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WebDDP with IC50 of 133.3 and 63.7 μM was obtained for A549/DDP cells with siNC or siUSP14#1 transfection, respectively (Figure 2A). DDP with IC50 of 57.7 and 86.2 μM was obtained for A549 cells with empty vector or USP14 expression vector transduction, respectively (Figure 2B). WebMar 22, 2024 · We developed CDDP-resistant A549 (A549/DDP) cells through prolonged incubation with the drug and performed RNA-seq on RNA extracts to determine …

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WebIC50 values of cisplatin were determined for A549/SEN and A549/CR cells using inhibition dose-response curves with variable slopes. For the colony formation assay, 500 cells were seeded in 12-well plates and cultured in … WebThis study was designed to define whether exposure to cisplatin could affect the sensitivity to EGFR TKIs because conflicting results have been presented. We established sublines that are resistant to cisplatin from EGFR wild-type cells (A549 and H460) and EGFR mutant cells (PC-9 and HCC827).

WebNov 2, 2015 · The IC 50 values of both curcumin and cisplatin in the A549 and H2170 cells were tested on IMR-90 cells to evaluate the toxic effect of curcumin and cisplatin on normal cells. IMR-90 cells were seeded …

Web0.744770. Filter: 760 entries 1 2 3 ... 31 CSV TAB XLS. The Genomics of Drug Sensitivity in Cancer Project is a collaboration between the Cancer Genome Project at the Wellcome … rawlings vapor helmet with maskWebAug 1, 2024 · A549 and A549/TAX cells were seeded in 100 mm cell plate, after culturing for 24 h, CHIP assay was conducted. CHIP was performed with rabbit smad3 antibody according to the manufacturer’s protocol (Millipore, USA). Then use the TIANGEN kit to extract DNA according to the instructions. PCR was used to test the performance of the … rawlings va countyWebJul 26, 2024 · In A549 cells, cisplatin exposure led to a significantly higher expression of genes coding for proteins mediating G2/M arrest and apoptosis (mouse double minute 2 … simple ground turkey seasoningWebFeb 4, 2024 · The IC50 concentration of cisplatin was required to achieve 50% of maximal growth inhibition after 72 h of exposure, and their standard errors were 0.82 ± 0.07 μM for A549 and 0.85 ± 0.06 μM ... simple ground turkey recipes healthyWebA cisplatin-resistant cell subline, HeLa/CDDP cells, showed a 19-fold resistance to cisplatin compared with the parent cells. The subline showed a collateral sensitivity to paclitaxel. An equitoxic dose (IC50) of cisplatin produced DNA fragmentation in HeLa cells but not in HeLa/CDDP cells. simple group costume ideasWebMar 27, 2024 · Cisplatin sensitivity (IC50) of the 9 NSCLC cell lines was determined using in vitro cytotoxicity assay (CCK-8 assay, Figure 1 (b) ). Based on the cut-off IC50 cisplatin concentration of 4 μ M, the cell lines were classified into cisplatin sensitive (IC50 ≤ 4 μ M) and resistant (> 4 μ M) groups. rawlings va weatherWebIC 50 s of oxaliplatin in HCT116 and A549 cancer cells in the absence and presence of WLR treatment Source publication +2 Aqueous extract of Lithospermi radix attenuates oxaliplatin-induced... simplegrout 180