Glut4 translocation assay

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August undefined, 2024

WebNov 7, 2010 · The translocation of GLUT4 from the cytoplasm to the plasma membrane can be visualized by immunocytochemistry, using fluorophore-conjugated GLUT4-specific antibodies. Here, we describe a... WebGlut4 translocation assays are usually performed in differentiated myocytes or adipocytes. The requirement for differentiation makes such assays difficult, particularly in high throughput screen (HTS) mode, since differentiation extends the assay time line and makes assays more variable from well to well and particularly from batch to batch of ...

A novel quantitative assay for analysis of GLUT4 …

WebDec 31, 2012 · TRF assays confirmed insulin-stimulated GLUT4 translocation, which can be inhibited by PI3K (phosphoinositide 3-kinase) or Akt [also called PKB (protein kinase B)] inhibitors. WebMay 13, 2024 · GLUT4 is the primary glucose transporter in adipose and skeletal muscle tissues. Its cellular trafficking is regulated by insulin signaling. Failed or reduced plasma membrane localization of... chisme no like youtubeelisa casa

Translocation and Redistribution of GLUT4 Using a Dual …

WebGlut4 Translocation Assay/Surface Glut4 Labeling. Cells expressing HA-Glut4/GFP were incubated for 2 h in low serum medium (LSM; DMEM complete medium and 0.5% fetal bovine serum). Cells were then incubated with or without 100 n m insulin for 45 min. To label surface Glut4, cells were rapidly chilled on an ice-water slurry and labeled with LSM ... WebThe agonist assay can be used to identify compounds that modulate translocation of GLUT4 and thereby affect glucose uptake. Insulin is used as reference agonist. Furthermore, the assay can potentially be formatted to screen for compounds that enhance the translocation of GLUT4 in response to insulin (i.e. insulin sensitizers). WebIt is crucial to determine the regulation of GLUT4 translocation and redistribution to the plasma membrane. The HA-GLUT4-GFP dual-reporter construct has become an important tool in the assessment of GLUT4 recycling in cultured adipocytes and myocytes. Through the use of light microscopy, this report … graphormer 代码讲解

Real Time Qualitative and Quantitative GLUT4 Translocation Assay

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WebFeb 10, 2013 · * Studied signaling mechanism of insulin-stimulated GLUT4 translocation in vitro * Studied the role of IKKepsilon on obesity/chronic … WebApr 1, 2005 · We have previously shown that insulin-stimulated GLUT4 translocation to the PM is blocked in 3T3-L1 adipocytes preincubated at 19 C ( 13 ). To study this translocation block in detail, we made use of a 96-well plate quantitative translocation assay recently developed in our laboratory ( 18 ). graph or military prosWebFeb 11, 2009 · GLUT4 (glucose transporter 4) plays important roles in glucose homoeostasis in vivo. GLUT4 expression and function are diminished in diabetic human and animal subjects. The goal of the present study is to develop a cell-based assay for identifying negative regulators of GLUT4 translocation as potential targets for the … graphor modeling

"WebApr 1, 2002 · Somwar, R. et al. GLUT4 translocation precedes the stimulation of glucose uptake by insulin in muscle cells: potential activation of GLUT4 via p38 mitogen-activated protein kinase. Biochem. " - Glut4 translocation assay

Glut4 translocation assay

A xanthene derivative, DS20060511, attenuates glucose ... - Nature

WebThe assay could collect real time data of GLUT4 translocation from thousand of cells/ sample and from many such samples in one experiment. We validated the assay using Insulin, insulin mimics ...

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WebOct 5, 2024 · Insulin stimulates glucose uptake in adipose tissue and skeletal muscle by inducing plasma membrane translocation of the glucose transporter GLUT4. Although the small GTPase Rac1 is a key regulator downstream of phosphoinositide 3-kinase (PI3K) and the protein kinase Akt2 in skeletal muscle, it remains unclear whether Rac1 also … WebAug 1, 2024 · Assessment of GLUT4 translocation in muscle and muscle cell lines has revealed insulin-stimulated increases at the cell surface of between two- and sixfold in human and rat muscles, respectively, in which the levels of cell surface GLUT4 were measured using affinity labelling approaches [29, 32, 33, 34 ].

WebGLUT4 (SLC2A4) is the insulin-responding glucose transporter, found predominantly in muscle cells and adipocytes (fat cells). After a meal, glucose that is absorbed from the digestive system and circulates in the blood now stimulates the release of insulin from the pancreas (Figure 4.10).This insulin is the signal for a rapid transfer of GLUT4 in muscle … WebMay 13, 2024 · At basal state, GLUT4 is primarily distributed on the membranes of the trans-Golgi network (TGN), endosomes and 50–70 nm tubulo-vesicular structures known as the GLUT4 storage vesicles (GSVs) 7,8.

WebJan 1, 2024 · GLUT4 translocation assay was carried on CHO-HIRC-myc-GLUT4 eGFP cell line; and a parallel glucose uptake study was carried out in differentiated 3T3L1 adipose and HepG2 cells. Briefly, a day before the experiment, 5 × 10 3 cells were plated in each well of a 96 well plate.

WebMar 27, 2024 · GLUT4 translocation In skeletal muscle, translocation and redistribution of GLUT4 to the plasma membrane is a characteristic feature in increased glucose uptake and the utilization effect of insulin. graphorn 5e statsWebDec 8, 2024 · It is crucial to determine the regulation of GLUT4 translocation and redistribution to the plasma membrane. The HA-GLUT4-GFP dual-reporter construct has become an important tool in the assessment of GLUT4 recycling in cultured adipocytes and myocytes. Through the use of light microscopy, this reporter construct allows for … chisme pronunciationWebA novel quantitative assay for analysis of GLUT4 translocation using high content screening. Insulin resistance is associated with obesity and can lead to several metabolic disorders including type II diabetes, nonalcoholic fatty liver disease and cardiovascular problems. chisme clothingWebJan 1, 2012 · Abstract. Insulin-stimulated glucose transporter 4 (GLUT4) translocation promoting glucose uptake is vital to glucose homeostasis and is a defined target of antidiabetic drug research. Existing functional assays to detect the process of GLUT4 translocation are hampered due to assay variability and low sensitivity, thus slowing … graphorn 5eWebJun 26, 2013 · Assays were performed 1–3 d after electroporation, as needed to achieve knockdown of the targeted protein as validated by Western blot. On the day of the assay, GLUT4 or TR translocation … chismerWebAug 20, 2024 · The xanthene derivative, DS20060511, is a skeletal muscle cell-specific inducer of GLUT4 translocation. We screened our chemical library, composed of more than 100,000 compounds, using L6-GLUT4myc ... chismes alamoWebOct 27, 2024 · Screening for compounds that induce the translocation of glucose transporter 4 (GLUT4) from the intracellular compartments to the plasma membrane in insulin-sensitive tissues is an innovative... graphorn breed