GS is subject to reversible covalent modification. Tyr of all 12 subunits can undergo adenylylation or deadenylylation by adenylyl transferase (AT), a bifunctional regulatory enzyme. Adenylylation is a post-translational modification involving the covalent attachment of AMP to a protein side chain. Each adenylylation requires an ATP and complete inhibition of GS requires 12 ATP. … WebMar 16, 2010 · The GS from Y30G01 and Y30G03 had a similar IC 50 for micafungin (138 and 385 mg/L, respectively) and for caspofungin (29 and 26 mg/L, respectively); these values were more than three log orders higher than those of the GS from TUA4 (Table 4). The inhibition curve for GS from the heterozygous Y30G strain was biphasic.
Controlling glutamine metabolism by acetylation Science …
WebAug 9, 2002 · GsCT expression inhibited beta (2)AR- and dopamine D (1A) receptor-mediated cAMP production, without affecting the cellular response to cholera toxin or forskolin, indicating that the peptide inhibited receptor-G (s) coupling without impairing G protein or adenylyl cyclase function. [35S]GTPgammaS loading of Galpha (q/11) by … WebGlufosinate acts as a glutamine synthetase (GS) inhibitor that causes elevated ammonia levels, but the mode of action of CT is unknown. GS has also been implicated in plant defense in certain plant-pathogen interactions. The effects of spray applications of glufosinate (1.0 mM) orbioherbicide (8.0 × 104 conidia ml-1), applied alone or in ... melissa ashworth midkiff
Long-acting capsid inhibitor GS-6207 confirms safety and
WebMar 1, 2003 · Recently, Coghlan et al. reported activation of GS in cells with selective low molecular weight organic GSK-3 inhibitor.In rat hepatoma cells, these compounds cause a reduction in the expression of the gluconeogenic enzymes phosphoenolpyruvate carboxykinase and glucose-6-phosphatase ().Potent and selective GSK-3 inhibitors, … WebApr 1, 1991 · Abstract. Glutamine synthetase (GS) utilizes various substituted glutamic acids as substrates. We have used this information to design herbicidal α- and γ-substituted analogs of phosphinothricin (l-2-amino-4-(hydroxymethylphosphinyl)butanoic acid, PPT), a naturally occurring GS inhibitor and a potent herbicide.The substituted … WebJan 6, 1998 · We have also assessed GTP[γS]-dependent inhibition of forskolin-stimulated adenylyl cyclase, which was unaffected (e.g., EC 50 = 42 ± 21 and 45 ± 17 nM, maximal … melissa ashworth blackstock